Scoring

There are four kinds of scores that Rock Maker displays:

 

Manual Scores

When you're viewing images, you can easily assign scores to your images by pressing the hotkey number on your keyboard (generally, 1-9, 9 high).

Scores tab

You can also assign images by clicking the score button on the Scores toolbar in the Images contextual tab set. You can customize many aspects about scores to fit your needs on the General setup page.

 

To score drops:

  1. While in Images view, select a drop by clicking it. If you want to assign the same score to multiple drops, select them all from the Canvas Tab.  (To open Images view, in the Experiment contextual tab set, click the View tab, and click the Image View button).
  2. Press a number on your keyboard to assign a score. Rock Maker moves you to the next image.

    OR

    Back in the Scores tab, click the desired score button. Rock Maker moves you to the next image.


Score buttons

Note: To assign a different score to a drop, simply type a different score from the same score group. If you have defined multiple score groups, you can assign one score from each group.

On the Drop tab, the score description appears on the bottom left.

On the Canvas tab, a plate's score appears in the upper-right corner of the image with its color as shown in the following example.

Plate's scores

 

Inherited Scores

If new images have been taken since the last scoring session, we call the score an inherited score, because the score was "inherited" from the previous session.

Tip: You can turn inherited scores on or off in System Properties by changing the "Experiment Score: Inherit Drop Score" value to True or False.

As you can see in the image below, an inherited score is outlined in the related score color.

Inherited score

Tip: Look for inherited scores to review new images. You may want to update the score.

Note: The score colors are associated with the number, not the shape.

 

Auto Scores

You can filter your drop images by their auto scores using the following buttons on the ribbon menu:

Auto Scores

Note: Auto scoring is a feature of Rock Imager 2.9. UV, UV-TPEF and SONICC images are analyzed by the Rock Imager Processor for crystals and then automatically scored as a positive or negative hit.

From left to right, these buttons are: the Auto Score Highlighter button, the Auto Score Filter: Any Hit button, and the Auto Score Filter: All Hits button.

For more information on what the three buttons do, see The Ribbon.

You can also adjust the thresholds for a positive hit for each imager on the Threshold Control (shown below), which will change how many of your drops are presented to you as positive hits, and how many are presented as negative hits.

Threshold Control

 

Computerized Scores

Computerized scores include auto scores from UV, UV-TPEF and SONICC, and FRAP data. Auto scores and FRAP data display with a black triangle over the upper-left portion of the score, as shown below. In this case, a score can be a plus or minus symbol when referring to UV, UV-TPEF and SONICC, or numerical data when referring to FRAP.

Computerized Scores

 

Related System Properties

System Properties in the Tools menu are how you change behind-the-scenes Rock Maker behaviors.

System Property Default Explanation
Experiment plate: Automatically move to next well after scoring (default) TRUE If set to TRUE, when you assign a score to an image Rock Maker will automatically advance to the next image.
Experiment Score: Inherit drop score TRUE Allows/disallows a score from an old image to be displayed as the score on a new image taken of the same drop.
Experiment Score: Restart from first well once the last is reached TRUE If set to TRUE, Rock Maker will bring you to the first well after you score the last well on a plate.
Scores: Auto Scores: Enabled TRUE If set to FALSE, Rock Maker will not display computerized autoscoring results for UV and/or SONICC images.
Scores: Auto Scores: FRAP Enabled FALSE If set to FALSE, Rock Maker will not display computerized autoscores for FRAP images.

 

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